Mechanical and optical properties have remained obstacles in design of the artificial cornea. In this project, we evaluated influence of collagen concentration on the regenerated cornea.The artificial cornea scaffold was prepared by collagen and NHS/EDC (N-hydroxysuccinimide/1-ethyl-3-(3-dimethylaminopropyl)carbodiimide).The concentrations of collagen in same dimensions were 10%, 12.5%, 15%, 17.5% and 20%, respectively. The transmissions of collagen with different concentrations were measured with UV-1800 in the wavelengths of 450nm, 500nm, 550nm, 600nm, 650n, 700nm. Mechanical properties for surgical substitution were evaluated in vitro through its ability to knot and bury with monofilament sutures (10-0).As the collagen concentration increased, the mechanical property of the cornea implant improved while optical property decreased. Transmission of the collagen in 10%, 12.5%, 15% concentrations were superior to the concentration of 17.5% and 20%. However, the former concentrations were easier to shear and tear during the knotting and burying process.The results demonstrate that collagen concentration has a correlation with mechanical properties of the cornea with decreased transmission. Further study will elucidate a collagen concentration that closely mirrors optical and mechanical properties of in vivo cornea.